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The route from Prague to Vienna is served daily by 153 bus departing from the stations of Prague Main Railway Station, Prague ÚAN Florenc, Prague Central Train Station (Wilsonova), Hlavní Nádraží - Wilsonova, Prague Florenc Central Bus Station, ÚAN Florenc, Prague Airport Terminal 1/2, Airport PRG Letiště Václava Havla, Prague Roztyly, Prague Vaclav Havel Airport Prague, Libeň and arriving to the stations of Vienna Erdberg, Vienna Airport, Vienna Main Train Station (Südtiroler Platz), Vienna Simmering Train Station, VIE Airport, Vienna Airport Bus Station, Vienna Central Train Station, Hauptbahnhof, Vienna Hbf Main Train Station (Südtiroler Platz), Vienna Schwechat Airport Busstation, Stadion Center, Vienna Stadioncenter.
The average duration of the journey is 5 h 39 min. The first bus leaves at 2:30am. The last is at 11:55pm.

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