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The route from Prague to Rome is served daily by 29 bus departing from the stations of Prague Main Railway Station, Prague Zličín, Prague Anděl, ÚAN Florenc, Prague Florenc Central Bus Station, Prague ÚAN Florenc and arriving to the stations of Rome Tiburtina Bus Station, Via Teodorico, Rome Tiburtina, Largo Guido Mazzoni, 3, Via Di San Pietro In Carcere, Rome Anagnina.
The average duration of the journey is 23 h 47 min. The first bus leaves at 12:10am. The last is at 11:55pm.

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