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The route from Prague to Madrid is served daily by 3 bus departing from the stations of Prague Florenc Central Bus Station, Prague, Florenc, ÚAN Florenc and arriving to the stations of Madrid Mendez Alvaro Estacion Sur, Madrid Est Sur, Estación Sur - Mendez Alvaro.
The average duration of the journey is 46 h 30 min. The first bus leaves at 5:00pm.

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