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The route from Pisa to Rome is served daily by 24 bus departing from the stations of Pisa, Pisa Pietrasantina Parking, Parcheggio Via Pietransantina, Pisa Pietrasantina Bus Station and arriving to the stations of Rome Fiumicino, Rome, Rome Anagnina, Rome Tiburtina, Largo Guido Mazzoni, 3, Via Teodorico.
The average duration of the journey is 6 h 37 min. The first bus leaves at 1:30am. The last is at 11:40pm.

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