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The route from Florence to Rome is served daily by 62 bus departing from the stations of Florence (Villa Costanza Tram T1), Florence (Piazzale Montelungo), Nord Autostazione, Terminal Bus Villa Constanza, Florence Bus Station, Florence Villa Costanza, Capolinea Tramvia Villa Costanza, Florence Train Station S. M. Novella, Busitalia Nord Autostazione and arriving to the stations of Rome Anagnina, Rome Tiburtina Bus Station, Largo Guido Mazzoni, 3, Via Teodorico, Rome Tiburtina, Via Tiburtina, Rome Fiumicino Airport.
The average duration of the journey is 4 h 10 min. The first bus leaves at 1:20am. The last is at 11:59pm.

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