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The route from Rome to Prague is served daily by 23 bus departing from the stations of Rome Fiumicino Airport, Rome Tiburtina Bus Station, Rome Tiburtina, Via Teodorico, Rome Anagnina and arriving to the stations of Prague ÚAN Florenc, Prague Main Railway Station, Prague Anděl, Prague Florenc Central Bus Station, ÚAN Florenc.
The average duration of the journey is 26 h 35 min. The first bus leaves at 8:55am. The last is at 11:35pm.

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