If the offers provided by buses do not suit your needs, trains and carpooling could represent a good alternative for this distance.
With such a long journey, we would like to give you some advice: you can look for a flight to Prague, or if your budget is limited, you can check night buses and trains to save the daytime for your visits.
How many bus connections exist to go from Rome to Prague per day?
What time do the busses to Prague leave?
While the first bus leaves at 08:09, the last one leaves Rome at 13:09. The duration of the journey is on average 21 hours and 2 minutes, but it can take you only 19 hours and 5 minutes with the fastest connections.
By bus from Rome to Prague: which companies offer the service?
With FlixBus you will be able to travel trom Rome to Prague by bus.
Flixbus is undoubtedly one of the youngest and most famous bus companies in whole Europe. With more than 40 millions passengers only in 2017, Flixbus offers national and international bus connections, also with night buses.