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The route from Brussels to Prague is served daily by 65 bus departing from the stations of Brussels South (Gare Du Midi), Brussels-North Station, Brussels Airport, Brussels North Train Station, Brussels Midi / Brussel Zuid East, Gare Du Midi, Gare Du Nord, Brussels North Station, Gare De Bruxelles-Nord and arriving to the stations of Prague ÚAN Florenc, Prague Anděl, Prague Florenc Central Bus Station, Prague Main Railway Station, ÚAN Florenc, Prague Wilsonova Bus Station, Hlavní Nádraží - Wilsonova, Prague ,Hradčanská.
The average duration of the journey is 19 h 30 min. The first bus leaves at 2:00am. The last is at 11:45pm.

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