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The route from Berlin to Prague is served daily by 201 bus departing from the stations of Berlin Südkreuz, Berlin SXF, Berlin Alt-Tegel, Berlin S-Treptower Park, Berlin Zoo, Berlin Alexanderplatz, Berlin TXL, E, Berlin Central Bus Station, ZOB, Südkreuz, Schönefeld SXF Airport, Berlin Schönefeld Airport, Berlin Wannsee and arriving to the stations of Prague Main Railway Station, Prague ÚAN Florenc, Prague Florenc Central Bus Station, Prague Airport Terminal 1/2, ÚAN Florenc, Airport PRG Letiště Václava Havla, Prague Anděl.
The average duration of the journey is 5 h 8 min. The first bus leaves at 12:10am. The last is at 11:59pm.

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